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標記抗體服務

 

       

 

服務宗旨:高品質的標記原料采購,規范的標記操作流程,嚴格的標記產品質控,合理的市場價格定位。
服務目標:以國際高標準的標記服務水平,提供高品質的標記抗體產品,助力科研人員輕松科研。



一、服務內容:

本服務項目主要為接收客戶的抗體、蛋白、多肽、小分子化合物進行常規標記物標記。

1. 可接收的待標記樣品包含抗體IgG、大分子蛋白、小分子多肽、小分子化合物等。

2. 可提供標記服務的標記物有:

A. 酶標記物:堿性磷酸酶(AP/ALP),辣根過氧化物酶(HRP);

B. 生物素(Biotin);

C. 常規熒光素:FITC,Rhodamine B(RBITC);

D. Cy系列熒光素:Cy3,Cy5,Cy5.5, Cy7;

E. Alexa Fluor系列熒光素:AF350,AF488,AF555,AF594,AF647,AF680,AF750;

F. 熒光蛋白:藻紅蛋白(PE),別藻藍蛋白(APC),PerCP;

G. 熒光雙標記:PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7;

H. 膠體金標記Co-Au;

二、服務流程:

1.  客戶提交委托標記請求并明確標記要求,與公司商定具體的實驗項目、數量和費用,雙方簽訂《委托標記制備合同》。

2.  客戶提供待標記樣品,我公司按照合同要求安排標記服務,并在規定時間內完成標記委托。

3.  標記完成后,我公司按照客戶要求分裝,免費低溫郵寄給客戶,同時提供標記報告。

三、樣品要求:

1.  待標記樣品需保證90%以上的純度,樣品中不含有干擾標記物結合的成分,如有特殊成分,請提前標注說明。

2.  待標記樣品最小標記量為1mg,最好是干粉,如果是液體,濃度應大于1mg/ml。

3.  抗體IgG樣品中應不含BSA、疊氮鈉、甘氨酸等成分。

4.  大分子蛋白應提供分子量、等電點、緩沖液成分及pH等信息;小分子多肽需提供氨基酸序列;小分子化合物還需提供分子結構式。

5.  請提前說明任何特殊情況。

四、收費標準:

1.  抗體和大分子蛋白標記收費標準:

 標記物  類型  標記物廠家  標記數量  周期
 1-2mg  2-5mg  5-10mg
 HRP    Sigma  1200  1600  2500  10天
 AP    Invitrogen  2000  2800  5000  10天
 Biotin  生物素  Sigma  1000  1500  2000  10天
 FITC  熒光  Sigma  1000  1500  2000  10天
 RBITC  熒光  Sigma  1200  1600  2500  10天
 Cy系列  熒光  Invitrogen  1500  2200  3200  10天
 Alexa Fluor  熒光  Life Technologies  2000  2500  4000  10天
 PE  熒光  GE  1600  2400  3200  10天
 APC  熒光  GE  1600  2400  3200  10天
 PerCP  熒光  GE  1600  2400  3200  10天
 PE-Cy雙標  熒光  GE& Invitrogen  3000  4800  6400  15天
 Co-Au  膠體金  Sigma  1500  2500  4000  10天

 

五、標記物介紹:
堿性磷酸酶(alkaline phosphatase,ALP或AP)是免疫診斷試劑產品最常用的標記酶之一,優點是穩定性高、靈敏度高。ALP是一種能夠將對應底物去磷酸化的酶,即通過水解磷酸單酯將底物分子上的磷酸基團除去,并生成磷酸根離子和自由的羥基,這類底物包括核酸、蛋白、生物堿等。而該脫去磷酸基團的過程被稱為去磷酸化或脫磷酸化。堿性磷酸酶是磷酸酶的一種,磷酸酶的作用與激酶的作用正相反,激酶是磷酸化酶,可以利用能量分子,如ATP,將磷酸基團加到對應底物分子上。

辣根過氧化物酶(Horseradish Peroxidase,HRP)活性高,穩定,分子量小,純酶容易制備,所以最常用。HRP廣泛分布于植物界,辣根中含量高,它是由無色的酶蛋白和棕色的鐵卟啉結合而成的糖蛋白,糖含量18%。HRP由多個同功酶組成,分子量為40,000,等電點為PH3~9,酶催化的最適PH因供氫體不同而稍有差異,但多在PH5左右。酶溶于水和58%以下飽和度硫酸銨溶液。HRP的輔基和酶蛋白最大吸收光譜分別為403nm和275nm,一般以OD403nm /OD275nm的比值RZ(德文ReinheitZahl)表示酶的純度。高純度的酶RZ值應在3.0左右(最高可達3.4)。RZ值越小,非酶蛋白就越多。

FITC和RhodamineB(RBITC)是常見的熒光素,性質較穩定;

Cy系列熒光染料由于熒光較強,對光猝滅有抵抗力而熒光不宜消退,近年來應用比較廣泛;

六、常用熒光素信息:


北京博奧森標記抗體發表文獻:

文獻名稱:The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression.

期刊名稱:Nature. 2016 Apr 14;532(7598):245-9. doi: 10.1038/nature17403. Epub 2016 Apr 6.

影響因子:(IF)41.456

產品應用:bs-5805R-FITC | RIPK-1 Polyclonal Antibody, FITC Conjugated | IHC-F

產品應用:bs-7596R-A647 | RIP3 Polyclonal Antibody, Alexa Fluor® 647 Conjugated | IHC-F

研究機構:S. Arthur Localio Laboratory, Department of Surgery, New York University School of Medicine; Department of Cell Biology, New York University School of Medicine; Department of Pathology, New York University School of Medicine; Cold Spring Harbor Laboratories.

文獻鏈接://www.ncbi.nlm.nih.gov/pubmed/27049944


文獻名稱:Protein kinase A determines timing of early differentiation through epigenetic regulation with G9a.

期刊名稱:Cell Stem Cell. 2012 Jun 14;10(6):759-70. doi: 10.1016/j.stem.2012.02.022.

影響因子:(IF)25.315

產品應用:bs-0008R-PE | Rabbit Anti-Nestin Polyclonal Antibody, PE Conjugated | FCM

產品應用:bs-2358R-PE | Rabbit Anti-FOXA2/HNF3 beta Polyclonal Antibody, PE Conjugated | FCM

研究機構:Department of Stem Cell Differentiation, Institute for Frontier Medical Sciences, Kyoto University; Laboratory of Reproductive Biology, Graduate School of Agriculture; Experimental Research Center for Infectious Disease, Institute for Virus Research,Kyoto University; Cancer Institute, Japanese Foundation for Cancer Research, Tokyo.

文獻鏈接://www.ncbi.nlm.nih.gov/pubmed/22704517


文獻名稱:Transcriptional profiling of mouse B cell terminal differentiation defines a signature for antibody-secreting plasma cells.

期刊名稱:Nat Immunol. 2015 Jun;16(6):663-73. doi: 10.1038/ni.3154. Epub 2015 Apr 20.

影響因子:(IF)24.973

產品應用:bs-9425R-PE | Rabbit Anti-TSPAN15 Polyclonal Antibody, PE Conjugated | FCM

研究機構:The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia; Department of Computing and Information Systems, The University of Melbourne; Department of Medical Biology, The University of Melbourne; Department of Pathology, The University of Melbourne; Department of Microbiology & Immunology, The University of Melbourne;

文獻鏈接://www.ncbi.nlm.nih.gov/pubmed/25894659


文獻名稱:Vagal Regulation of Group 3 Innate Lymphoid Cells and the Immunoresolvent PCTR1 Controls Infection Resolution.

期刊名稱:Immunity. 2017 Jan 17;46(1):92-105. doi: 10.1016/j.immuni.2016.12.009. Epub 2017 Jan 5.

影響因子:(IF)24.08

產品應用:bs-6505R-A647 | Rabbit Anti-15 Lipoxygenase 1 antibody,Alexa Fluor®647 Conjugated | FCM

研究機構:Center for Experimental Therapeutics and Reperfusion Injury, Harvard Institutes of Medicine, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA; Lipid Mediator Unit, Centre for Biochemical Pharmacology, William Harvey Research Institute, Barts and the London School of Medicine, Queen Mary University of London, London EC1M 6BQ, UK.

文獻鏈接://www.ncbi.nlm.nih.gov/pubmed/28065837


文獻名稱:Pten loss in the bone marrow leads to G-CSF-mediated HSC mobilization.

期刊名稱:J Exp Med. 2013 Oct 21;210(11):2337-49. doi: 10.1084/jem.20122768. Epub 2013 Oct 14.

影響引子:(IF)13.912

產品應用:bs-2478R-PE | Rabbit Anti-CCL12/MCP5 Polyclonal antibody, PE Conjugated | FCM

研究機構:Deutsches Krebsforschungszentrum (DKFZ); Heidelberg Institute for Stem Cell Technology and Experimental Medicine; Swiss Institute for Experimental Cancer Research (ISREC), School of Life Science, Ecole Polytechnique Fédérale de Lausanne (EPFL); Department of Molecular and Medical Pharmacology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research; Department of Hematology, Universit?tsklinikum Essen, Westdeutsches Tumorzentrum (WTZ); Department of Laboratory Medicine and Comprehensive Cancer Center, University of California

文獻鏈接://www.ncbi.nlm.nih.gov/pubmed/24127490


文獻名稱:Identification of blood vascular endothelial stem cells by the expression of protein C receptor.

期刊名稱:Cell Res. 2016 Oct;26(10):1079-1098. doi: 10.1038/cr.2016.85. Epub 2016 Jul 1.

影響引子:(IF)12.41

產品應用:bs-4800R-A647 | Rabbit Anti-NG2 Polyclonal antibody,Alexa Fluor® 647 Conjugated | FCM

研究機構:The State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

文獻鏈接://www.ncbi.nlm.nih.gov/pubmed/27364685


文獻名稱:Endothelial to mesenchymal transition is common in atherosclerotic lesions and is associated with plaque instability.

期刊名稱:Nat Commun. 2016 Jun 24;7:11853. doi: 10.1038/ncomms11853.

影響引子:(IF)11.33

產品應用:bs-5760R-A647 |Rabbit Anti-FAP1Polyclonalantibody,ALexaFluor® 647 Conjugated | FCM

產品應用:bs-5758R-A647 |Rabbit Anti-FAPAPolyclonalantibody,ALexaFluor ® 647 Conjugated | FCM 

研究機構:The Zena and Michael A. Wiener Cardiovascular Institute, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA. Department of Genetics and Genomic Sciences and Icahn Institute for Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.Trialomics LLC, Seattle, Washington 98115, USA. Department of Pathology and Immunology, Washington University, St Louis, Missouri 63110, USA. Brigham and Women's Health Care, Boston, Massachusetts 02115, USA. Marie-Josée and Henry R. Kravis Cardiovascular Health Center, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA. Centro Nacional de Investigaciones Cardiovasculares (CNIC), 28029 Madrid, Spain. Center for Molecular Medicine, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, 20892, USA.

文獻鏈接://www.ncbi.nlm.nih.gov/pubmed/27340017



文獻名稱:Macrophage-derived extracellular vesicle-packaged WNTs rescue intestinal stem cells and enhance survival after radiation injury.

期刊名稱:Nat Commun. 2016 Oct 13;7:13096. doi: 10.1038/ncomms13096.

影響引子:(IF)11.33

產品應用:bs-1948R-Biotin | Rabbit Anti-WNT5A polyclonal antibody, BiotinConjugated | ELISA

產品應用:bs-1933R-Biotin |Rabbit Anti-WNT9Apolyclonalantibody, BiotinConjugated| ELISA

研究機構:Department of Radiation Oncology, Albert Einstein College of Medicine &Montefiore Medical Center, Bronx, New York 10461, USA. Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA. Department of Medicine, Division of Digestive and Liver Diseases, Irving Cancer Research Center, Columbia University, New York, New York 10032, USA. Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas 66160, USA. Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, 10461, USA. Department of Pathology, Albert Einstein College of Medicine, Bronx, New York 10461, USA. MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh EH16 4TJ, UK

文獻鏈接://www.ncbi.nlm.nih.gov/pubmed/27734833



文獻名稱:A TNFRSF14-Fc?RI-mast cell pathway contributes to development of multiple features of asthma pathology in mice.

期刊名稱:Nat Commun. 2016 Dec 16;7:13696. doi: 10.1038/ncomms13696.

影響引子:(IF)11.33

產品應用:bs-2462R-PE |Rabbit Anti-TNFSF14 polyclonal antibody, PEConjugated | FCM

研究機構:Department of Pathology &Department of Genetics &Department of Pediatrics &Department of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, Stanford University School of Medicine, Stanford, California 94305, USA. Department of Immunology, Unit of Antibodies in Therapy and Pathology, INSERM U1222, Institut Pasteur, Paris 75015, France. CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences and Department of Medicine I, Research Laboratory of Infection Biology, Medical University of Vienna, Vienna 1090, Austria. Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, La Jolla, California 92037, USA. Institute for Immunology, Technische Universit?t Dresden, Dresden 01307, Germany.

文獻鏈接://www.ncbi.nlm.nih.gov/pubmed/27982078


文獻名稱:HCV induced reduction in miR‐181a impairs CD4+ T cell responses via over‐expression of DUSP6.

期刊名稱:Hepatology. 2015 Apr;61(4):1163-73. doi: 10.1002/hep.27634. Epub 2015 Feb 4.

影響引子:(IF)11.19

產品應用:bs-11546R-FITC |Rabbit Anti-DUSP6Polyclonalantibody, FITCConjugated | FCM

研究機構:Center for Inflammation, Infectious Disease and Immunity, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN.

文獻鏈接://www.ncbi.nlm.nih.gov/pubmed/25477247



文獻名稱:Neutralization of pro‐inflammatory monocytes by targeting TLR2 dimerization ameliorates colitis.

期刊名稱:EMBO J. 2016 Mar 15;35(6):685-98. doi: 10.15252/embj.201592649. Epub 2016 Feb 15.

影響引子:(IF)10.43

產品應用:bs-2716R-PE |Rabbit Anti-TLR6Ployclonalantibody,PEConjugated| FCM

研究機構:Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, Israel. Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel. Department of Biological Services, The Weizmann Institute of Science, Rehovot, Israel.  Department of Veterinary Resources, The Weizmann Institute of Science, Rehovot, Israel. 

文獻鏈接://www.ncbi.nlm.nih.gov/pubmed/26884587  


文獻名稱:Multistage NanovehicleDelivery System Based on Stepwise Size Reduction and Charge Reversal for Programmed Nuclear Targeting of Systemically Administered Anticancer Drugs.

期刊名稱:Advanced Functional Materials.25(26).DOI: 10.1002/adfm.201501248.July 8, 2015

影響引子:(IF)10.40

產品應用:bs-0293G-FITC |Goat Anti-rat IgG antibody, FITCConjugated | Other

研究機構:Key Laboratory of Drug Targeting and Drug Delivery System, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu, P. R. China 

文獻鏈接://onlinelibrary.wiley.com/doi/10.1002/adfm.201501248/pdf



文獻名稱:Therapeutic effects of glatiramer acetate and grafted CD115+ monocytes in a mouse model of Alzheimers disease.

期刊名稱:Brain. 2015 Aug;138(Pt 8):2399-422. doi: 10.1093/brain/awv150.Epub 2015 Jun 6.

影響引子:(IF)10.23

產品應用:bs-0397R-A488 |Rabbit Anti-MMP9Plyclonalantibody,Alexa Fluor®488 Conjugated | FCM

研究機構:Department of Neurosurgery, Maxine-Dunitz Neurosurgical Institute, Cedars-Sinai Medical Centre, Los Angeles, CA 90048, USA.Widjaja Foundation Inflammatory Bowel and Immunobiology Research Institute, Cedars-Sinai Medical Centre, Los Angeles, CA 90048, USA. Department of Biomedical Sciences, Cedars-Sinai Medical Centre, Los Angeles, CA 90048, USA

文獻鏈接:莱斯特城赛程



 

法律聲明:The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc. U.S. Patent No. 5,675,063.LINK

 

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